dpp-4 assay buffer  (Bachem)

Journal: Cardiovascular Diabetology

Article Title: Dipeptidyl peptidase-4 inhibition with linagliptin prevents western diet-induced vascular abnormalities in female mice

doi: 10.1186/s12933-016-0414-5

Figure Lengend Snippet: Linagliptin (LGT) has neutral effects on body weight and aortic advanced glycation end-products (AGE). a WD feeding for 4 months resulted in significant weight gain in both the cohorts. b Plasma DPP-4 activity was significantly decreased with LGT treatment. c AGE immunostaining in aorta was significantly increased in WDC. LGT treatment did not decrease it significantly. Quantification and representative images shown. Values are mean ± SE. CDC control diet control, CDL control diet linagliptin, WDC western diet control, WDL western diet linagliptin. Post-hoc comparisons within a time point; *p < 0.05 CDC vs WDC; # p < 0.05 CDC vs CDL; † p < 0.05 WDC vs WDL. Scale bars represent 50 mμ

Article Snippet: 20 μL plasma was diluted in DPP-4 assay buffer and substrate, 200 M H-Ala-Pro-AFC (I-1680; Bachem), was added.

Techniques: Activity Assay, Immunostaining, Western Blot

Journal: Cardiovascular Diabetology

Article Title: Dipeptidyl peptidase-4 inhibition with linagliptin prevents western diet-induced vascular abnormalities in female mice

doi: 10.1186/s12933-016-0414-5

Figure Lengend Snippet: WD feeding causes a , b peri-aortic fibrosis and ( c , d ) medial thickening which is ameliorated by the DPP-4 inhibitor, LGT. a Picro sirius red and b Verhoeff-von Gieson staining. Values are mean ± SE. CDC control diet control, CDL control diet linagliptin, WDC western diet control and WDL western diet linagliptin. Post-hoc comparisons within a time point; *p < 0.05 CDC vs WDC; † p < 0.05 WDC vs WDL

Article Snippet: 20 μL plasma was diluted in DPP-4 assay buffer and substrate, 200 M H-Ala-Pro-AFC (I-1680; Bachem), was added.

Techniques: Staining, Western Blot

Journal: Cardiovascular Diabetology

Article Title: Dipeptidyl peptidase-4 inhibition with linagliptin prevents western diet-induced vascular abnormalities in female mice

doi: 10.1186/s12933-016-0414-5

Figure Lengend Snippet: WD feeding induced aortic oxidative stress is ameliorated with DPP-4 inhibition. a 3-nitrotyrosine staining; b EC 3-nitrotyrosine; c VSMC 3-nitrotyrosine. Values are mean ± SE. CDC control diet control, CDL control diet linagliptin, WDC western diet control and WDL western diet linagliptin. Post-hoc comparisons within a time point; *p < 0 0.05 CDC vs WDC; † p < 0.05 WDC vs WDL

Article Snippet: 20 μL plasma was diluted in DPP-4 assay buffer and substrate, 200 M H-Ala-Pro-AFC (I-1680; Bachem), was added.

Techniques: Inhibition, Staining, Western Blot

Journal: Cardiovascular Diabetology

Article Title: Dipeptidyl peptidase-4 inhibition with linagliptin prevents western diet-induced vascular abnormalities in female mice

doi: 10.1186/s12933-016-0414-5

Figure Lengend Snippet: WD feeding induced changes in FGF-23 and Klotho expression are restored by DPP-4 inhibition. a FGF-23 staining; b Endothelial FGF-23; c Adventitia FGF-23; d Endothelial klotho staining; e Endothelial klotho staining f Adventitia klotho staining; Average gray intensities in the different cohorts. Values are mean ± SE. CDC control diet control, CDL control diet linagliptin, WDC western diet control and WDL western diet linagliptin. Post-hoc comparisons within a time point; *p < 0 0.05 CDC vs WDC; † p < 0.05 WDC vs WDL; # p < 0.05 CDC vs CDL. Scale bars represent 50 mμ

Article Snippet: 20 μL plasma was diluted in DPP-4 assay buffer and substrate, 200 M H-Ala-Pro-AFC (I-1680; Bachem), was added.

Techniques: Expressing, Inhibition, Staining, Western Blot